Abstract
Black Sbank disease of tobacco, Nicodanae tabacum, caused by Pkytopathora nicotianae, is one of the most eve of soil borne diseases. The present investiption was initiated to study the relative importance of various inducible mechanisms operative in this plant:pathogen interaction. Tobacco cell is culture were treated with beat-released soluble cell-wall elicitors from myceiial walls of the pathogen. The timing and intensity of the induced defence responses of the cells with regard to the synthesis of pbytoalexins, lignin and defence hydrolases were evaluated. An elicitor concentration of 40 μg ml −1 resulted in the optimal induction of sesguiterpenoid pbytoslexin synthesis which was detectable 3–4 hr after elicitation and reached a maximum at 20–28 hr post-elicitation. Increased lignin deposition was detectable 4 hr after elicitation with maximal synthesis between 12 and % hr. Induction of extraceffrdar and cellular β-1,3-glucauases was observed within 4 hr, exhibiting a biphasic response with an initial peak at 8–12 h. Increased cellular and echltimse activities were detected from 8 and 16 hr onwards, respect ively. These results were supported by enzyme staining of electropboretk separated isoforms. The rests obtained indicate that the elicitation of tobacco cells by P. nicotianae derived sigioal molecules exhibits the properties of a multicomponent dynamic system with different protective mechanisms wing complementary roles in the overall expression of the defence response.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: The International Journal of Biochemistry & Cell Biology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
