Abstract
BackgroundThe long-lasting and abundant blooming of Pelagia noctiluca in Tunisian coastal waters compromises both touristic and fishing activities and causes substantial economic losses. Determining their molecular mode of action is, important in order to limit or prevent the subsequent damages. Thus, the aim of the present study was to investigate the propensity of Pelagia noctiluca venom to cause oxidative damage in HCT 116 cells and its associated genotoxic effects.ResultsOur results indicated an overproduction of ROS, an induction of catalase activity and an increase of MDA generation. We looked for DNA fragmentation by means of the comet assay. Results indicated that venom of Pelagia noctiluca induced DNA fragmentation. SDS-PAGE analysis of Pelagia noctiluca venom revealed at least 15 protein bands of molecular weights ranging from 4 to 120 kDa.ConclusionOxidative damage may be an initiating event and contributes, in part, to the mechanism of toxicity of Pelagia noctiluca venom.
Highlights
The long-lasting and abundant blooming of Pelagia noctiluca in Tunisian coastal waters compromises both touristic and fishing activities and causes substantial economic losses
Inhibition of cell proliferation Cytotoxic effects of P. noctiluca crude venom on HCT 116 cells after 24 h incubation was measured by MTT assay
Measurement of reactive oxygen species (ROS) production To check the oxidative stress status in HCT 116 cells in response of crude venom of P. noctiluca at different concentrations (80, 160, 320, and 640 μg/ml) corresponding to inhibitory concentration of 50% of cell viability (IC50)/4, IC50/2, IC50, and 2 × IC50 s of crude venom), we measured the production of fluorescent dichlorofluorescein product (DCF)
Summary
The long-lasting and abundant blooming of Pelagia noctiluca in Tunisian coastal waters compromises both touristic and fishing activities and causes substantial economic losses. Determining their molecular mode of action is, important in order to limit or prevent the subsequent damages. One of the most distinctive aspects of jellyfish physiology is related to its biologically active components and organelles contained in specialized cells called nematocysts. They are located along the tentacles and body. Electrophoretical analyses recognized eight different fractions, distinguished by molecular mass [15]
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