Abstract
BackgroundCytokine production and histopathological changes occur in the lungs of mice after intranasal inoculation with Campylobacter jejuni, but the levels of cytokines in different organs to which C. jejuni disseminates have not been studied.FindingsAdult BALB/c mice were intranasally inoculated with C. jejuni 81–176 (test) or phosphate-buffered saline (control) (n=16 per group). The levels of cytokines in the organs (spleen, liver, and small and large intestines) to which C. jejuni disseminated were measured by ELISA. Two cytokine patterns were observed. First, increased proinflammatory cytokines, TNF-α, IL-1, and IL-2, were followed by anti-inflammatory cytokines, IL-4 and IL-10 in the spleen and large intestine. Second, in the liver and small intestine, there was a predominant production of anti-inflammatory cytokines, IL-4 and IL-10, with some increase in IL-2 levels. In the spleen and intestines, the levels of pro- and anti-inflammatory cytokines were concurrently increased.ConclusionDissemination of C. jejuni is associated with the production of different cytokine profiles in different tissues, with the proinflammatory response appearing in the spleen and large intestine at an earlier time point than in the liver and small intestine. The organs produce different cytokine profiles in response to C. jejuni dissemination. These preliminary findings should be confirmed with a study involving a larger group of animals.
Highlights
MethodsA lung model of C. jejuni infection was used in adult mice [18,19]
Cytokine production and histopathological changes occur in the lungs of mice after intranasal inoculation with Campylobacter jejuni, but the levels of cytokines in different organs to which C. jejuni disseminates have not been studied
Dissemination of C. jejuni is associated with the production of different cytokine profiles in different tissues, with the proinflammatory response appearing in the spleen and large intestine at an earlier time point than in the liver and small intestine
Summary
A lung model of C. jejuni infection was used in adult mice [18,19]. Adult BALB/c mice were intranasally inoculated with ~4 × 109 cfu (colony forming units) of C. jejuni strain 81–176 (n=16, test) or phosphate-buffered saline (PBS, pH 7.2) (n=16, control). After 1–10 days, blood and several organs (spleen, liver, and small and large intestines) of mice (from 4 mice each from test and control groups sacrificed at each time point) were collected, weighed and placed on ice. The organs were homogenised in PBS using a pestle and mortar under sterile condition in a biosafety cabinet. Homogenates were resuspended in 1% Triton X-100 (Sigma, St. Louis, MO, USA) on ice and centrifuged at 4°C at 19,319 × g for 10 min in a Beckman J2-MI centrifuge using a JA 20.1 rotor (Beckman, Fullerton, CA, USA).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
