Abstract
Polyclonal antibodies (PAb anti-perch P450 1A) raised against the cytochrome P450 1A (P450 1A) orthologue in perch were used to quantify hepatic P450 1A in fish species used or proposed to be used in Swedish environmental biomonitoring programs. Protein blots revealed that the PAb anti-perch P450 1A recognized one major β-naphthoflavone-inducible microsomal protein band in all studied fish species (feral perch, pike, dab and blenny, and the hatchery-reared rainbow trout and lake trout). All feral fish exhibited elevated P450 1A levels, with the highest levels found close to industrial or municipal discharges. The present study confirms earlier findings indicating that immunochemical assays can be used to detect the induction of P450 1A in environmental biomonitoring.
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