Abstract
Aerobic glucose dissimilation of washed cells ofStreptococcus lactis grown in peptone-glucose-yeast extract medium is characterized by the formation of large amounts of lactic acid, a small amount of acetic acid, and traces of acetoin: a corresponding amount of oxygen is taken up. Aerobic metabolism by washed cells ofS. lactis andLeuconostoc mesenteroides is far more oxidative when the cells have been grown on peptone-glucose-yeast extract agar supplied with 10 ppm of hemin than when they have been grown in the absence of hemin. In the former case respiration is strongly inhibited by KCN and only slightly by bis(tributylgermanium) oxide, (Bu3Ge)2O. Respiration of cells grown without hemin, on the other hand, is strongly inhibited by (Bu3Ge)2O but only moderately by KCN. In cells grown in the presence of hemin, spectra of ana2- andb-type cytochrome were recognized but not in cells grown without hemin. The NADH-oxidase activity of such cells is not affected by KCN.
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