Induction of chromosome rearrangements in a 4H(4D) wheat-barley substitution using a wheat line containing aPhsuppressor gene

Abstract

The aim of the experiments was to develop translocation lines by inducing homoeologous chromosome pairing in a 4H(4D) wheat-barley substitution line previously developed in Martonvasar. It was hoped to incorporate various segments of the barley 4H chromosome from the 4H(4D) substitution into wheat. Observations were made on the frequency with which wheat-barley translocations appeared in the F 2 progeny grains from a cross between the line CO4-1, which carries the Ph suppressor gene from Aegilops speltoides and thus induces a high level of homoeologous chromosome pairing, and the 4H(4D) wheat-barley substitution line, and on which chromosome segments were involved in the translocations. The translocations were identified by means of genomic in situ hybridisation. Of the 117 plants examined, three (2.4 %) were found to contain translocations. A total of four translocations were observed, as one plant contained two different translocations. The translocations consisted of one centric fusion, two dicentric t...