Induction of chromosome aberrations and sister-chromatid exchanges in CHO-K1 cells by o-phenylphenol

Abstract

o-Phenylphenol (OPP, is used in Japan as a fungicide in food additives for citrus fruits. The induction of chromosome aberrations and sistem-chromatid exchanges (SCEs) by OPP in cultured Chinesse hamster ovary (CHO-K1) cells was studied. Cells were exposed to various concentrations of OPP ranging from 50 to 175 μg/ml for 3 h, and further incubated for 27 and 42 h. These incubation periods are almost equal to 2 and 3 cell cycles. SCEs and chromosome aberrations were induced by OPP at concentrations of 100, 125 and 150 μg/ml after the incubation for 27 h. For chromosome aberrations, chromatid breaks and exchanges there was a dose-dependent increase. Diplochromosomes due to endoreduplication were also caused bu the same concentrations of OPP in a dose-dependent manner. After incubation for 42 h, chromosome aberrations were also increased by OPP at concentrations of 100 and 125 μg/ml, but the frequencies of SCEs were not significantly different from those of the control. These results suggest that OPP has a cytogenetic toxicity, and that the DNA damage resulting in SCEs induced by OPP is relatively short-lived and can be required during the longer incubation time.