Abstract

Potato and corn starch granules were crosslinked by epichlorohydrin to various extents (DC = 0.6 - 7.1). Most of these crosslinked starches retained a granular structure after autoclaving, though their crystalline structure had been lost. Using these starches as a carbon source for the cultivation of Bacillus circulans F-2, the digestion of starch granules, bacterial growth and production of amylase were investigated in comparison with those obtained with raw potato and corn starch granules. An increase in the DC of the starches decreased their degradation rate and bacterial growth. On the other hand, the amount of amylase produced increased with an increase in the DC of the added starches. CLP induced a larger amount of amylase than CLC of the same DC. CLP (DC = 4.0) induced 0.6U/ml of amylase, which is 1.8 times as much as that induced by raw potato starch. This, together with the fact that it is autoclavable, proved CLP to be a most excellent carbon source for the production of Bacillus circulans F-2 amylase.

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