Abstract
We investigated the cytotoxicity of six sesquiterpenes (igalane, 5-epoxyalantolactone, 5-epoxy-1(10),11(13)-germacradiene-8,12-olide, 11,13-dihydroalantolactone, isoalantolactone, alantolactone and 11, 13-dihydroisoalantolactone) isolated from Inula helenium against murine hepatoma cell lines (Hepa 1c1c7 cells and it mutant type BPRc1 cells). All the components tested in the study showed strong cytotoxic activity against both hepa1c1c7 and BPRc1 cell lines. In particular, 5-epoxyalantolactone and alantolactone showed higher cytotoxic activity for both cell lines than the others. Flow-cytometry analysis showed that 5-epoxyalantolactone and alantolactone induced the accumulation of the sub-G1 peak and increased the portion of apoptotic annexin V positive cells in a dose-dependent manner, suggesting apoptotic activity of the compounds. In conclusion, 5-epoxyalantolactone and alantolactone present in Inula helenium appear to be major components responsible for induction of apoptotic cell death.
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