Abstract
Cutaneous melanoma is one of the most aggressive forms of skin cancer. Arctigenin, one of the major bioactive compo-nents of Arctii Fructus, has been reported to exhibit antioxidant, antitumor and anti-inflammatory activities. In the pre-sent study, we investigated the effect of arctigenin on induction of apoptosis in highly metastatic SK-MEL-28 human melanoma cells. Arctigenin inhibited growth of SK-MEL-28 cells in a dose-dependent manner. Treatment of SK-MEL-28cells with arctigenin caused cleavage of caspases 3, 7 and 9, and poly (ADP-ribose) polymerase in a dose-dependent manner. Furthermore, acetylation of histone H3 and H4 in the SK-MEL-28 cells was dramatically increased by arctigenin treatment. Collectively, these findings indicate that arctigenin-induces apoptosis of SK-MEL-28 melanoma cells via activation of caspases and histone acetylation.
Highlights
IntroductionDecades of epidemiological studies have established a direct association between consumption of plant-based diets and a reduction in risk of cancer [1,2,3,4]
We investigated the effect of arctigenin on induction of apoptosis in highly metastatic SK-MEL-28 human melanoma cells
To determine the effect of arctigenin on cell viability, SK-MEL-28 cells were treated with the varying concentrations of arctigenin for 48 hours, and the cell viability was evaluated by the MTT assay
Summary
Decades of epidemiological studies have established a direct association between consumption of plant-based diets and a reduction in risk of cancer [1,2,3,4]. Efforts directed at the identification of bioactive ingredients in those diets and delineation of their mechanism(s) have demonstrated that even ubiquitous, non-nutritional secondary plant metabolites, such as flavonoids and polyphenolics widely present in foods consumed in the US, have significant health consequences [5,6,7,8,9,10]. Arctigenin, one of the major bioactive components from the fruits of the burdock, has been reported to exhibit antioxidant, antitumor and anti-inflammatory activities as a phenylpropanoid dibenzylbutyrolactone lignan [11,12,13]. Arctigenin occurs in a variety of plants including Bardanae fructus (Goboshi), Saussurea medusa (Compositae), T. nucifera and tropical climbing shrub Ipomea cairica [14,15,16,17]
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