Abstract
A primary immune response was obtained with suspensions of spleen cells in vitro and the polymer of Salmonella adelaide flagellin as antigen. This response is assayed by a technique which uses bacterial immunocytoadherence as a method of measuring the number of antibody-producing cells. The number of antibody-forming cells was shown to increase with time to a peak at 4 days and the system was antigen-dose dependent; the optimal amount being 0.002-0.02 μg, per ml. of culture fluid. Concentrations of 3 μg. per ml. of antigen caused specific unresponsiveness of cell-cultures, which, as critical tests indicated, was the induction of tolerance in vitro.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
