Abstract
INTRODUCTIONActivated dendritic cells (DCs) are the most efficient antigen-presenting cells (APCs) in the immune system. This protocol describes three different methods to activate antigen-specific CD4+ T cells using (i) bone-marrow-derived DCs, (ii) endogenous dermal DCs that drain to the local lymph node (LN), and (iii) tissue-resident APCs in a delayed-type hypersensitivity (DTH) response.
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