Abstract

A cDNA clone, designated AF19-1, was isolated from a cDNA library derived from insulin-stimulated hepatoma cells. The nucleotide sequences of AF19-1 showed 83% homology to Alu consensus sequence. It contained a full-length 300-bp Alu family sequence followed in direct tandem by a partial sequence of Alu left monomer. Primer extension analysis confirmed that this Alu transcript was induced even in short-term insulin stimulation. The increase in Alu-transcripts in the early phase of insulin stimulation in hepatoma cells suggests that the Alu sequences may play some important regulatory roles in gene expression.

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