Abstract
SD rats were pretreated with whole body hyperthermia (rectal 42°C) for 15min. The level of calcitonin gene-related peptide (CGRP) in plasma, and α- and β-CGRP mRNA as well as heme oxygenease-1 and heme oxygenase-2 mRNA in dorsal root ganglia were determined by radioimmunoassay and semi-quantitative reverse-transcription polymerase chain reaction, respectively. Heat stress induced only the expression of α-CGRP or heme oxygenease-1 but not β-CGRP or heme oxygenase-2 mRNA, and the release of CGRP and induction of α-CGRP mRNA expression were abolished by pretreatment with Zinc protoporphyrin IX, the heme oxygenase inhibitor, or methylene blue, the inhibitor of soluble guanylate cyclase. These results indicate that induction of α-CGRP mRNA expression in rat DRG by heat stress involves the heme oxygenase-1/carbon monoxide pathway.
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