Abstract
Bipolaris sorokiniana causes spot blotch, an important disease of wheat in Asia and America. To understand the host response to challenge by B. sorokiniana, we calculated the plant disease index (PDI) of twelve popular wheat (Triticum aestivum L.) genotypes and examined the expression levels of PR protein genes and wax synthesizing genes on an inoculation time-course using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Gene expression was studied in a resistant (R) and a highly susceptible (HS) genotype selected from a PDI experiment under glasshouse conditions. A gene (β-1,3-glucanase II) was induced at 24 h post inoculation and to higher levels in the resistant genotype Chiriya-3 (PDI = 1.3) than in the highly susceptible genotype Agra Local (PDI = 40.1). Densitometry analysis showed that expression of an Actin gene was the same at all stages of inoculation due to its constitutive expression in both the resistant and susceptible genotypes, while a 7-fold increase in expressi...
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