Abstract
Artificially inducing 2n gametes through chromosome doubling is an effective way to obtain polyploids. In this study, Eucalyptus urophylla microsporogenesis and flower development were investigated to guide 2n pollen induction. We also investigated suitable conditions for colchicine treatment. Our results showed that E. urophylla 2n pollen was spherical and had a large volume (mean diameter 28.57 ± 0.46 μm), while normal untreated pollen (mean diameter 19.68 ± 0.11 μm) was tetrahedron. The highest rate of 2n pollen production was 28.71 % when the flower buds, which ranged in size from 3.5 to 4.0 mm, underwent treatment with 0.5 % colchicine solution for 6 h. Further studies suggested that diplotene to diakinesis and metaphase I to telophase I were suitable meiotic stages for chromosomes doubling, due to asynchronous development of microsporogenesis between the anthers in a single flower bud. These data help to illuminate research in other areas, such as triploid eucalyptus production by chromosome doubling of female gametes.
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