Abstract
Effects of prostaglandin A 2 (PGA 2) on glutathione (GSH) status in L-1210 cells were examined. When the cells were cultured in the presence of PGA 2, a persistent rise of cellular GSH concentration was observed 6 h after the addition of PGA 2. This stimulatory effect of PGA 2 was abolished if the cells were pretreated with an enzyme inhibitor of GSH synthesis, buthionine sulfoximine. Subsequent study with cell free extract of cultured L-1210 has revealed that PGA 2 stimulated the biosynthesis of γ-glutamylcysteine synthetase (EC 6.3.2.2). Actinomycin D inhibited this stimulatory effect of PGA 2 on cultured cells. The optimal pH, Km value for glutamic acid and sensitivity to inhibitors of γ-glutamylcysteine synthetase from PGA 2 treated and nontreated cells were virtually the same. Thus, our findings suggest that PGA 2 induced γ-glutamylcysteine synthetase in cultured L-1210 cells which is responsible for the elevated level of GSH in these cells.
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