Abstract
Stylosanthes guianensis is an elite and important forage legume species, which is extensively cultivated in tropical areas. Polyploid breeding via exposure to colchicine is a conventional and practical method to improve varieties of S. guianensis. Terminal buds of S. guianensis Reyan No.5 seedlings were treated with different concentrations of colchicine (0.00, 0.05, 0.10, 0.15, 0.20, and 0.25%) for 24, 48, and 72 h. Morphological and cytological variants were observed at a frequency of <96% among transplanted seedlings. The cytogenetic analysis of young leaf cells was conducted on all variants to identify their ploidy levels. The most efficient procedure for tetraploid production was the treatment of seedling apical buds with 20% colchicine for 48 h, with the tetraploid induction rate being 10%. This is a relatively simple and reliable method for the production of tetraploidy in S. guianensis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.