Abstract
Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ‘Permata’) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.
Highlights
Tito et al (2011) reported that tomato cell culture extract contains antioxidant compounds such as fla-Secondary plant products have been considered vonoids, hydroxycinamic acid, glycoalkaloid, phenolas one of the main sources of pharmaceuticals, flavor, ic acid, and terpenoids
Previous studies have found that how their biosynthesis and how to improve the prod- tomato callus extract has cytoprotective activity in ucts by in vitro culture technologies have been exten- preventing H2O2-induced on Vero and Human Dermal sively studied since the 1960s (Bourgaud et al, 2001)
Callus induction Ten days old in vitro grown seedlings were used as the source of explants
Summary
Secondary plant products have been considered vonoids, hydroxycinamic acid, glycoalkaloid, phenolas one of the main sources of pharmaceuticals, flavor, ic acid, and terpenoids This extract contains agrochemicals, nutrition, and industrial biochemical. Plant cell cultures have been widely known as tech- (Hana, 2016; Prastowo, 2017; Utama, 2018; Dewi, nique for accommodating the production of large- 2018; Rumiyati et al, 2018). This extract is rich in scale high-value metabolites by cost-effective using a polyphenols and flavonoids with low antioxidant acbioreactor (Georgiev et al, 2009). Callus derivatives that play a role in cell regeneration cultures can be sub-cultured to another medium to (Rumiyati et al, 2018)
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