Induction and evaluation of murine colitis induced by T cell transfer.

Abstract

Inflammatory bowel diseases (IBD) involve repetitive bouts of inflammation in the intestinal tract and can result in severe morbidity for patients. Moreover, long-standing IBD increases the risk for developing intestinal neoplasia. Although several factors including immune cell activity, microbiota and diet have been implicated in IBD pathogenesis, it is still considered a disease of idiopathic origin. Therefore, much work is needed to identify the critical mediators in IBD onset, severity and response to treatment. Mouse models are useful for identifying factors that contribute to IBD and the efficacy of therapy, which can then be tested in humans. There are currently multiple IBD models including the use of chemical induction, genetic manipulation and modulation of the immune response. The T cell transfer colitis model provides a quality mimic of human IBD that is T cell driven and results in inflammation in both the ileum and colon. Here, we have provided a detailed step-by-step protocol to induce inflammation and assess disease severity using this model. Such a detailed methodologic description will help to increase its utilization to advance IBD research.