Abstract
The papaya (Carica papaya L.) is a tropical fruit tree grown in many coastal region of Colima. The experiments were conducted to optimize cultural requirements for induction and culture of shoot of C. papaya cv. Red Lady in vitro. The crown, fruits, and leaves were removed from 1-year-old trees in the field for stimulate growth of lateral shoots with application of 289 μM gibberellic acid solution along steam surface. Apex (2 × 2 mm) of the shoots induced were established in Drew and Smith (DS) medium containing 1 μM 6-benzylaminopurine (BAP) and 1 μM naphthaleneacetic acid (NAA). After 90 days of establishment, the explants were transferred to a multiplication medium consisting of DS medium containing different concentrations of BAP and NAA (0:0, 2:0.1, 2:0.5, 2.5:0.5 and 3:0.5 μM, respectively). Individual shoots were excised from the proliferating cultures and subcultured onto fresh medium at 30- to 40-day intervals. The shoot number induced of trees in the field was of 47.5 per plant. After three subculture of shoot the best multiplication factor per subculture (6.9) was when 2 or 2.5 uM of BAP and 0.5 μM of NAA were added to DS medium.
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