Abstract
Tetraploid poplar plants were induced by colchicine from tissue-cultured shoots; the induction efficiency varied with colchicine concentration. Morphologically, induced tetraploid plants were significantly distinguishable from diploid plants; their leaves were larger, thicker, and rounder, with larger stomata and more chloroplast numbers but smaller stomata index in the abaxial leaf epidermis. Data from validation with chromosome counting and flow cytometry corresponded well with changes in stomata size and chloroplast number. This ready use protocol would facilitate poplar breeding. Tetraploid induction by colchicine in Populus euroamericana from tissue cultured shoots.
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