Abstract
Hypoxanthine-phosphoribosyltransferase negative (Hprt<sup>–</sup>) cell lines derived from an Akodon cursor liposarcoma were obtained by induced mutagenesis. All but one Hprt<sup>–</sup> cell line lacked Hprt mRNA transcripts while one (AKO 3) coded for a truncated protein. Cell fusion and karyotypic analyses showed that one cell line (AKO 1-15) could be successfully used for constructing hybrid panels and allow for a clear identification of human chromosomes in hybrid cells.
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