Abstract
Meiosis in Saccharomyces cerevisiae can be induced by deprivation of nutrients. Here, we present a protocol for inducing synchronous meiosis in SK1, the most efficient and synchronous yeast strain for meiosis, by exposing SK1 cells to liquid medium that contains potassium acetate as a nonfermentable carbon source and lacks nitrogen. These synchronous meiotic yeast cultures can be subjected to a range of molecular and cytological analyses, making them useful for investigating the genetic and molecular determinants of meiosis.
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