Inducible IL-10 secreting CD49b+Treg cells as cell based-therapy for rheumatoid arthritis�

Abstract

Event Abstract Back to Event Inducible IL-10 secreting CD49b+Treg cells as cell based-therapy for rheumatoid arthritis’ Julie QUENTIN1* 1 INSERM, France Adoptive transfer of Tregs is a promising approach to restore tolerance in autoimmune disease. However several subsets of Tregs need to be precisely defined to clearly establish which Tregs will be able to dampen efficiently the immune response in the various settings. We previously showed the potential of IL10-secreting induced CD49b+ Tregs to protect and prevent an experimental model of arthritis. Nevertheless the optimal dose, the phenotype and the in vivo-suppressive mechanism of these Tregs remain unknown. Here, we investigated and compared the therapeutic potential of CD25+FoxP3+ and induced CD49b+ FoxP3- Tregs in experimental models of arthritis or inflammation. Tregs were generated in naïve mice following repetitive injections of immature DCs. Tregs purification was based on the negative selection of CD4 T cells isolated from spleen and liver of vaccinated mice. Several doses of CD49b+ Tregs were intravenously injected at day 28 in established arthritis. We observed that the injection of 105 CD49b+ or CD25+ cells reverse clinical symptoms of arthritis while interestingly, a lack of efficacy of higher doses was observed. In vitro suppressive experiments confirmed the similar efficiency of both populations and phenotype analyses of CD49b Tregs showed expression profile of several Tregs specific markers (LAP+, LAG+, CTLA-4high). Moreover, in an OVA-specific model of inflammation, we demonstrated the higher impact of the CD49b Tregs on the proliferation of effector cells. Altogether, our results confirm the therapeutic potential of IL-10 secreting T cells in experimental model of arthritis in curative settings and unravel their mechanism of suppression. Keywords: regulatory T cells, Arthritis, Rheumatoid, mouse models, Dendritic Cells, Inflammation Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: QUENTIN J (2013). Inducible IL-10 secreting CD49b+Treg cells as cell based-therapy for rheumatoid arthritis’. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00640 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Julie QUENTIN, INSERM, MONTPELLIER, France, julie.quentin@inserm.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Julie QUENTIN Google Julie QUENTIN Google Scholar Julie QUENTIN PubMed Julie QUENTIN Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.