Abstract
Repeated administration of gold (chloroauric acid) to rats resulted in a marked increase in the level of low molecular weight renal metal binding proteins (RMBP). [ 35S]Cysteine incorporation studies pointed to an induced biosynthesis of the latter under the influence of gold. The gold-binding proteins (Au-BP) were found predominantly in the kidney cytosol, but also extracts from 12,000 g and 1000 g sediments (mitochondria and nuclei) contained low molecular weight gold complexes. The Au-BP isolated from the post-mitochondrial supernatant of rat kidneys had an apparent molecular weight of 12,000, and on DEAE-cellulose gave four fractions all containing gold. in amounts 11–33 μg Au/mg protein, and copper, above 18 μg/mg protein. The protein solutions were yellowish and their u.v.-spectra showed extinction maxima at 220–225 nm as well as slight shoulders at 250–280 nm. The Au-BP displayed a tendency for coprecipitation and aggregation. A relation of the renal Au-BP to the mercury- and bismuth-binding proteins is suggested.
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