Abstract
Background3-Hydroxypropionic acid (3-HP) is an important platform chemical that boasts a variety of industrial applications. Gene expression systems inducible by 3-HP, if available, are of great utility for optimization of the pathways of 3-HP production and excretion.ResultsHere we report the presence of unique inducible gene expression systems in Pseudomonas denitrificans and other microorganisms. In P. denitrificans, transcription of three genes (hpdH, mmsA and hbdH-4) involved in 3-HP degradation was upregulated by 3-HP by the action of a transcriptional regulator protein, LysR, and a cis-acting regulatory site for LysR binding. Similar inducible systems having an LysR transcriptional regulator were identified in other microorganisms that also could degrade 3-HP. A docking study showed that the 3-HP binding pocket is located between the N-terminal helix-turn-helix motif and the C-terminal cofactor-binding domain.ConclusionsThis LysR-regulated 3-HP-inducible system should prove useful for control of the level of gene expression in response to 3-HP.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-015-0353-5) contains supplementary material, which is available to authorized users.
Highlights
Screening of 3‐HP‐inducible promoters in P. denitrificans 3-Hydroxypropionic acid (3-HP) is a carbon compound not commonly encountered in the natural environment; neither its use as a carbon substrate nor its biological degradation has been adequately elucidated
In the presence of 3-HP, two different LysR-family transcriptional regulators, designated C3- and C4-LysR, respectively, were found to stimulate the transcription of the catabolic genes hpdH, hbdH and/or mmsA involved in 3-HP degradation
The 3-HP-inducible systems promise to be of a great utility to the development of gene expression systems that are regulated by 3-HP
Summary
We report the presence of unique inducible gene expression systems in Pseudomonas denitrificans and other microorganisms. In P. denitrificans, transcription of three genes (hpdH, mmsA and hbdH-4) involved in 3-HP degradation was upregulated by 3-HP by the action of a transcriptional regulator protein, LysR, and a cis-acting regulatory site for LysR binding. Similar inducible systems having an LysR transcriptional regulator were identified in other microorganisms that could degrade 3-HP. A docking study showed that the 3-HP binding pocket is located between the N-terminal helix-turn-helix motif and the C-terminal cofactor-binding domain
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