Abstract
The Ca. LSU intron flanking a 129 bp exon up stream and a 100 bp exon downstream was inserted into thelacZ gene on pRS126 to transformE. coli, Northern blot analysis and RT-PCR showed that splicing of Ca. LSU inE. coli is efficient upon inducible expression of the precursor RNA. In contrast, co-transcriptional self splicing of the intronin vitro is much less active. Therefore, thisE. coli splicing system can be used as a better model to investigate the effect of the ribozyme inhibitors on Ca. LSU splicing in living cell. We examined the effects of neomycin sulfate and pentamidine on Ca. LSU splicing inE. coli, and found that these drugs does dependently inhibit the intron splicing. However, heomycin is more potent than pentamidine in this action.
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