Induced-orientation of nitrogen monoxide and azide ion vibrations in human hemoglobin in bidistilled water solution under a static magnetic field.

Abstract

In this study, we report the effects of static magnetic fields (SMFs) at 200 mT on different hemoglobin aqueous solutions, in the absence and in the presence of sucrose and trehalose, studied by FTIR spectroscopic techniques. Significant decrease in intensity of Amide I and Amide II vibration bands was observed after 6 h exposure for hemoglobin in bidistilled water solution. Also, it was observed that the decrease in intensity of the Amide I band was larger than the Amide II after exposure. This result can be explained assuming that an SMF induces increase of hydrogen bonding in hemoglobin in bidistilled water solution. In particular, the use of second-derivative analysis highlighted two absorption peaks at 1907 and 2022 cm-1 that can be attributed to nitrogen monoxide vibration and antisymmetric stretch of azide ion bound, respectively. These vibrations increased significantly after exposure to the SMF (P < 0.01). This result can be explained assuming that exposure to an SMF induces the orientation of nitrogen monoxide and azide ion ligands toward the direction of the field. Finally, it was observed that the addition of sucrose and trehalose in hemoglobin aqueous solution inhibited such alterations, suggesting that bioprotective effectiveness of these disaccharides occurs after exposure to an SMF. Bioelectromagnetics. 38:447-455, 2017. © 2017 Wiley Periodicals, Inc.