Abstract
With 3 figures and 2 tables AbstractGroundnut is an important oilseed crop grown extensively in tropical and subtropical regions in nearly 100 different countries. Monounsaturated fatty acid like oleic acid is known to have beneficial health effects. Hence, recent research efforts are directed towards an improvement of oleic acid in groundnut genotype, TFDRG 5. In the present study, 47 true breeding mutants with various morphological traits were isolated at M3 through gamma rays and/or sodium azide mutagenesis. These mutants were characterized for oil and fatty acid compositions at M8 and M9 generations. Of them, TGM 192M had nearly 27.3% improvement in oleic acid content which in turn increased the oleic/linoleic acid (O/L) ratio to 5.5 from 2.0 in parent. Sequencing of mutated ahFAD2A gene detected several point mutations in the coding region. Restriction digestion with Hpy99I and in silico translation of the mutated sequence further confirmed the G to A transition is responsible for high oleate trait in this mutant seed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
