Abstract
Abstract During ovulation, granulosa cells and cumulus cells synthesize and secrete a wide variety of factors via the exocytosis system. Exocytosis is controlled by the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex consisting of proteins residing in the vesicle membrane and the plasma membrane. SNAP25 localizes on the plasma membrane whereas synaptotagmins (SYT) reside in secretion vesicle membranes. After Ca2+-induced binding of SNAP25 and SYT, the release of vesicle-contained factors is triggered. Therefore, we sought to determine if induction of Syt mRNA and binding of SYT to SNAP25 in granulosa and cumulus cells were involved in exocytosis during ovulation. After hCG stimulation, the expression levels of Syt1, Syt2, Syt4 and Syt6 were induced markedly in granulosa cells, whereas the levels of Syt3 and Syt7 mRNAs did not change dramatically. The levels of SYT1 protein were also increased 4 hr after hCG injection and remained increased until 16 hr post-hCG...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
