Abstract
Current procedures for induced spawning in milkfish, Chanos chanos, involve the injection of pituitary homogenate and human chorionic gonadotropin followed by hand stripping of the hydrated eggs. This procedure results in the loss of valuable broodstock, low fertilization rates and unpredictable time of spawning. A reliable method for inducing spawning is essential. In this study, luteinizing hormone-releasing hormone analogue (LHRH-a) was tested for its effectiveness as an ovulatory and spawning agent. A single administration of LHRH-a via pellet implantation or liquid injection induced spontaneous spawning approximately 48 or 24 h after application, respectively. The average dosage used was 41.7 ± 3.3 μg/kg body weight for pellet implant and 58.7 ± 9.3 μg/kg body weight for injection. The spontaneous release of eggs was achieved in all spawning attempts when the initial egg diameter was at least 800 μm on average and had a single mode distribution. Seven of 15 attempts with natural fertilized spawns were successful and resulted in an average of 83.8% fertilization.
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