Induced resistance in tomato plants against root knot nematode using biotic and abiotic inducers

Abstract

A greenhouse experiment was conducted in the experimental farm station of plant pathology research institute during 2015 season at three times of application of biotic and a biotic elicitors , to evaluate the efficient antagonistic bacterial strains Bacillus subtilis, Serratia marcescens, Cyanobacterial strain Spirulina platensis and silver nanoparticles (AgNPs) against root-knot nematode Meloidogyne incognita infecting tomato plants. Nematode reproduction, Plant growth characters, endogenous hormones, anti-nematocidal protein markers as response to induction of SAR in leaves was recorded. Data recorded that using Bacillus subtilis at the same application time of M. incognita recorded 86% and 88% reduction of root galls and egg masses respectively. All tested elicitors appeared significant reduction in nematode reproduction. Also data revealed that treated tomato plants with tested elicitors recorded more significant values of growth characters. This was the fact when being compared with those of non-treated plants. Moreover, data revealed that contents of both GA and IAA were markedly decreased in infected plants than that of healthy ones. At the same time, marked increases in the contents of JA and ABA were observed in infected plants as being compared with healthy ones. On the other hand, results showed different responses as regards the determined hormones due to the application of different used elicitors. On the other hand, the results appeared that, a new pattern of proteins were produced, as well as, different increasing in the density of bands according to the type of used elicitors and also to t he time of application.