Abstract
Gametogenesis is cytologically affected by a large number of genes. Mutations induced in these genes by mutagenic environmental hazards such as gamma rays, chemical such as EMS may cause anomalies that may appear in form of pollen sterility. Due to effect of maximum dose of combination treatment of gamma rays and ethyl methane sulfonate (500Gy+ 0.5% EMS) applied on the seeds of Linum usitatissimum L., there is appearance of phenomenon known as chromosome elimination which is resulted in form of small microcytes resulting into sterile pollen grains. Migration of micronuclei towards the wall of microspore and its separation as a bud or microcyte might be the possible cause of pollen sterility.
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