Abstract
Discovery of induced Pluripotent stem cells (iPSCs) has revolutionized the fields of stem cell biology and regenerative medicine. iPSCs can now be generated through reprogramming of somatic cells from various tissues and mammalian species by ectopic expression of defined factors (Oct3/4, Sox2, c-Myc, Klf4). However, these methods of gene transduction, often involving viral vectors for delivery and genomic integration, may not be safe for clinical application sowing to high rates of malignant transformation and tumor genesis. In this article we provide a comprehensive overview of various new strategies including but not limited to mRNA or protein based delivery and/or chemical induction of pluripotency that circumvent the use of viral vectors for gene delivery. These methodological advancements increase the ease and efficiency of reprogramming, avoiding genomic modification, thereby increasing the suitability for clinical and translational applications in humans.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
