Induced of Systemic Resistance in Tomato Plant Against Tomato Bushy Stunt Virus by Bion and Riboflavin

Abstract

The aim of this study was to evaluate the efficiency of abiotic agents Bion and Riboflavin in inducing resistance in tomato plant Geesy cultivar against Tomato bushy stunt virus (TBSV), under plastic house conditions. The experiment was included two application with induce resistance agents: Bion & Riboflavin used by two concentration for foliar spray before and after 3,6 and 2,4 days artificial inoculation with virus. The treatment was evaluated by using several parameters such as estimation of percentage and severity of disease infection, serological evaluation by DAS-ELISA, estimation of peroxidase absorbance change and some of the plant growth parameters. The best treatment was foliar spray with Bion 1 mg/ml before 6 days of virus inoculation showed reduction in the percentage of disease infection which was 37.1% compare with control treatment, on the other hand above treatment was caused high inhibition activity of virus (TBSV) multiplication which was 70% as demonstrated by ELISA absorbance values 0.296 nm compare with control treatment which inoculated with virus only was 0.972 nm. Results also indicated that the treatment by spray Riboflavin concentration 10 mM before (4 days) the inoculation of virus was inhibition of percentage of disease was 35%. On the other hand the treatment was used of Riboflavin 10 mM spray gave significant increase in the activity of peroxidase after 14 days of virus inoculation which was give 71.172 absorbance compare with control treatment which was 28.193.).