Abstract
A simple one-test quantitative determination has been developed for volatile bioactive compounds. It may be applied to any visible response induced in any organism grown in solid gel cultures and it is, therefore, potentially widely applicable. The bioactivity determined is the induction of sexual reproduction (selfing) in Phytophthora cinnamomi-A 2 compatibility type by a volatile component of culture filtrates of Trichoderma koningii. Ether extraction of the active compound(s) and recovery on solvent evaporation was found to be efficient. The previously known 6-pentyl-α-pyrone from T. viride was isolated from T. koningii and found to be inactive, and 6-[pent-1-enyl]-α-pyrone from T. viride, previously reported associated with activity, was not detected associated with the activity from T. koningii following chromatography.
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