INDUCED HETERODUPLEX GENOTYPING OF TNF-α, IL-1β, IL-6 AND IL-10 POLYMORPHISMS ASSOCIATED WITH TRANSCRIPTIONAL REGULATION

Abstract

We describe the construction and use of 7 induced heteroduplex generators, reagents for the rapid and unequivocal genotyping of nucleotide sequence polymorphism in TNF-α, IL-1β, IL-6 and IL-10. Polymorphisms detected are those previously associated with regulation of gene transcription: TNF-α positions −308 and −238; IL-1β position +3953; IL-6 position −174; and IL-10 positions −1082, −819 and −592. The reagents were used for analysis of allele and haplotype frequencies in a population of healthy Caucasian volunteer blood donors.