Induced desynaptic variation in poppy (Papaver somniferum L.)

Sana Naseem,Girjesh Kumar

doi:10.1590/s1984-70332013000400007

Sana Naseem, Girjesh Kumar

Open Access

https://doi.org/10.1590/s1984-70332013000400007

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Abstract

Cytological investigation of EMS (ethyl methane sulphonate) treated population demonstrated enhanced univalent frequency per cell with unequal separation at Anaphase I. In contrast to controlled plants, medium strong type desynaptic plants were obtained from 0.6 % EMS treated set, revealing high frequency of univalents at Metaphase I, along with bivalents, which were loosely paired. The univalents remained unpaired till the end of meiosis, leading to formation of micronuclei and abnormal tetrads. These plants had high pollen inviability and sterile seeds. It might be possible that EMS had acted on some genes responsible for chiasma formation, resulting in early chiasma dissociation, which suggests that EMS can act as a potential tool in the development of male sterile lines. The study demonstrated the feasibility of chemical mutagenesis in mutation breeding programme on poppy (Papaver somniferum L.).

Highlights

Meiosis is a specialized differentiation process that generates recombinant haploid gametes from a diploid zygote (Pankratz and Forsburg 2005)
The absence or failure of synapsis is termed as asynapsis, whereas the immediate separation of the homologues following normal pachytene pairing is specified as desynapsis (Gottschalk and Kaul 1980a, b)
Both spontaneous and induced types of desynapsis have been reported in different plant species like Pennisetum ramosum (Jauhar et al 1971), Zinnia haegena (Singh and Gupta 1981), Capsicum annum (Rao and Kumar 1983), Hordeum vulgare (Srivastava 1974, Kumar and Singh 2002), Oryza sativa (Reddi and Rao 2000), Cicer arietinum (Kumar and Sharma 2001), Anogeissussericea (Rao and Kumar 2003), Glycine max (Palmer and Horner 2000, Bione et al 2002, Kumar and Rai 2006), Corchorus fascicularis

Summary

Introduction

Meiosis is a specialized differentiation process that generates recombinant haploid gametes from a diploid zygote (Pankratz and Forsburg 2005). Meiotic recombination has been frequently analysed through cytological and genetical methods. Mutation in the genes directing meiotic recombination may cause failure or early termination of chiasma formation.

Results

Conclusion