Abstract
Hyaluronic acid-acridine orange complexes in water show a symmetric doublet CD band centered at 455 nm. The exciton-like CD band indicates a preferred left-handed chirality for this polymer in solution, which correlates with the proposed left-handed double helical structure of hyaluronic acid in oriented film. The sign and general shape of the induced CD band with hyaluronic acid (dye concentration 2.5×10 −5M) is the same throughout the range of polymer-dye ratio studied. The single CD band which appears at a 10 −4M concentration of dye has been attributed to dye-dye interaction. Hyaluronic acid, preheated to 95°C for one hour, followed by rapid cooling, does not show any induced CD band.
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