Abstract
I WAS interested in Mr. T. Goodey's letter under the above heading in NATURE of March 13, but should like to make a few remarks thereon. Hay infusion, which Mr. Goodey states caused the excystation of Colpoda cucullus, is prepared from dried grass, and here we have the products of cytolysis, and, in consequence, should expect the presence of auxetics. As a matter of fact, any vegetable infusion contains auxetics, the presence of which can be demonstrated by the jelly method on human lymphocytes, as described by Dr. H. C. Ross, “Induced Cell Reproduction and Cancer” (London: John Murray, 1910). Encysted forms of Colpoda cannot be compared with the winter spores of Polytoma, as in Colpoda there is, so far as I can gather from Mr. Goodey's letter, no conjugation prior to the encystment, and consequently no real development is necessary for the excystation, but only rupture of the cyst-wall. In Polytoma, however, the cytoplasm within the spores has to undergo several complex changes, leading ultimately to division of sarcode, formation of envelopes round the products of division, and the development of flagella. Thus, I take it that in Colpoda there is no reproductive process in the excystation, and consequently no necessity for auxetics; anything that will cause the rupture of the cyst-wall being sufficient, although, as already shown, auxetics were certainly present in the hay infusion.
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