Abstract

Research on spawning stimulation of Sahyadria denisonii was carried out from June 2022 to October 2022 at the Research and Development Center for High-Tech Agriculture with three inducing agents, HCG, Ovaprim, and LHRHa + Domperidone. Each concentration of a hormone used in the experiment was applied on 5 pairs of brood fish. A 1 ml syringe was used for injecting to the anterior region of the dorsal fin by using a single-injection method and the injection timing of fish was 18 hours. Results showed that Ovaprim had an effect on inducing ovulation and laying eggs at a dose of 0.4-0.5 ml/kg of female fish. Female fish were exposed to hormones and ovulated after 11 hours 25 minutes to 11 hours 28 minutes from the time of injecting, 80% reproduction rate, actual fecundity from 747.67 to 750.33 eggs/female, 84% fertilisation rate, 84% hatching rate, time of embryo development was 30 hours at a water temperature of 29oC. The average length of new-hatched larvae was 3.2 mm with a large amount of yolk sac, the time to consume the yolk was from 3 to 4 days. HCG and LHRHa + Domperidonedid not have any effect on ovulation and spawning in fish.

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