Abstract

Oxyeleotris marmorata was induced to ovulate using human chorionic gonadotropin, followed by successful artificial fertilization. The mean diameter of the unfertilized egg was 0.83 mm. The egg elongated on contact with water with an increase in length of about 53%. Over 90% of the eggs were fertilized, of which over 90% hatched subsequently. Hatching time was extremely variable, ranging from 2 to 5 days after fertilization, when the eggs were hatched in petri-dishes at a temperature of 27±1°C, but it was fairly constant at around 3 days after fertilization when the eggs were hatched in continuously aerated and filtered water at a constant temperature of 27°C. The larvae did not survive for more than a few days. This problem of larval mortality is discussed. The sex differentiation and the embryonic development of the fish are described.

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