Indonesian

Abstract

Anwar S, Putra AC, Wulandari AS, Said S. 2016. The 5’ untranslated region of thyroglobulin gene (TG5) polymorphism in Bali cattle (Bos javanicus). Pros Sem Nas Masy Biodiv Indon 2: 159-164. Intramuscular fat or also called as ‘marbling’ is one of the essential components in producing premium meat quality in beef cattle. The polymorphism of 5’ untranslated region of thyroglobulin gene (TG) at locus 5’UTR|BstYI (TG5) have been widely used as a genetic marker to improve marbling and to eat quality in some beef cattle breeds. The objective of this study was to identify the genetic polymorphism of TG5 in Bali cattle (Bos javanicus). A total of one hundred DNA samples of Bali cattle used in this study were from Maiwa Breeding Center (MBC) area of Enrekang district, South Sulawesi province. The method used in the present study was PCR-RFLP using BstYI restriction enzyme. The result of this study showed that only two types of genotypes were found, including CC and TT with the respective proportions of 99% and 1%. The allelic frequencies of C and T were 0.995 and 0.005, respectively. The chi-square test showed that the genotypic frequencies of TG5 in the population tested were in Hardy-Weinberg equilibrium (HWE). The observed (Ho) and expected (He) heterozygosity values were 0.010 and 0.009, respectively. The value of polymorphism information content (PIC) was low (0.009). Finally, it could be concluded that the TG5 marker was uninformative and may not be used as a genetic marker in Bali cattle from MBC area. Additional samples from another region as Bali cattle breed resources outside the MBC area are needed to provide clearer information of the TG5 polymorphism on Bali cattle in Indonesia.