Abstract

Yulistia L, Munawar, Widjajanti H. 2015. Bacterial biodiversity of coal ash waste from industrial cooking oil in Kumai, Central Kalimantan. Pros Sem Nas Masy Biodiv Indon 1: 1302-1306. The research has studied the biodiversity of bacteria in coal ash waste from palm oil manufactory in Kumai, Central Kalimantan on medium with carbon source glucose, sucrose, starch, mannitol and acetic acid. The research was conducted in November 2014-February 2015, at the Laboratory of Microbiology Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University, South Sumatra. The purpose of this research is to determine the bacterial diversity that is at waste coal ash, which is expected to be the basis for the development of coal ash waste processing biologically so environmentally friendly. This research was conducted through several phases, namely, isolation, purification, selection, characterization, identification, calculation of the amount of bacterial cell density and diversity index calculations bacteria contained in waste coal ash. The results obtained in this study is 45 bacterial isolates from five sources of carbon used, and 45 of the bacterial isolates included into three genera of bacteria namely Aeromonas sp., Branhamella sp. and Nisseria sp. The highest diversity index of bacterially derived from medium with a carbon source from sucrose 2.35; then 1.63 high from acetate acid, 1.27 high from starch, 1.07 and 1.00 moderately from glucose and mannitol. The conclusion of this research is the best carbon source used to grow bacteria from waste coal ash is sucrose; genera of bacteria obtained at the stage of identification that is Aeromonas sp., Branhamella sp. and Nisseria sp.

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