Abstract
A study has been conducted to screen and multiply calluses and buds of tobacco transformed with the Soybean Mosaic Virus (SMV) coat protein gene and to induce them to plantlet formation by using MS medium with phytohormon treatments. The multiplication of calluses and buds was carried out by sub-culturing on MS medium using 0.3 mg/L NAA+1 mg/L BAP, followed by induction of plantlet formation on MS medium supplemented with NAA and BAP at varied concentration. The results showed that tobacco calluses transformed with SMV coat protein gene was multipliable on MS medium supplemented with NAA at a concentration of 0.3 mg/mL and BAP at 1 mg/L concentration. In this experiment kanamycin was used at a concentration of 100 I¼g/mL which resulted in the viability level of 84%. On MS medium supplemented with 0.3 mg/L NAA, 0.1 mg/L BAP, and 100 I¼g/mL kanamycin, induction of calluses to plantlet formation reached 20% level.
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