Indonesia

<p><em>The objective of this study were to find out and to compare the effect of supplementation of L-carnitine and tuna fish oil and lemuru fish oil on fatty acid saturated, fatty acid non saturated concentration and peroksida, TBA number.</em></p><p><em> The materials used were 20 male PE (Peranakan Etawah) goats of 2 until 3 month old and 15.5-23.5 kilogram body weight. The feedstuff used were wild grass, yellow corn, rice bran, soy bean meal, coconut meal, mineral premix. L-carnitine, tuna fish oil and lemuru fish oil. The method of the research was experimental in vivo using Completely Randomized Blok Design. There were 4 treatments in each treatment and 5 replication. Treatment consisted of P<sub>0</sub>= control ration, P<sub>1</sub>=P<sub>0</sub> +L-carnitine 50 ppm, P<sub>2</sub>=P<sub>1</sub> + tuna fish oil 5 ml or equal with 4% in the ration, and P<sub>3</sub>=P<sub>1</sub>+ lemuru fish oil 5 ml or equal with 4% in the ration. The variables measuared fatty acid saturated, fatty acid non saturated concentration and peroksida number..</em></p><p><em> The results of variance analysis showed that the effect of supplementation of tuna fish oil and lemuru fish oil (P<sub>2 </sub>and P<sub>3</sub>) in the rations contain 50 ppm L-carnitine were significantly (P<0.01) on faty acid saturated, faty acid non saturated concentration and peroksida number.</em></p><p><em>The conclusion of this research shows that the supplementation of 50 ppm L-carnitine, tuna and lemuru fish oil 5ml that was equal to 4% in goat rations can incrase non saturated fatty acid from 59,74 to 67,36%, than to decreased saturated fatty acid from 40,26 to 32,64 %. Proces burned of meat goat to increase peroxida from 0.1437 until 0.1615 miliequivalen/kg and malonaldehyde from 1,807 until 2,440 mg/kg then to decrease concentration omega-3 fatty acid from 5,957 until 5,945%.</em></p><p><em> </em></p><p><em>Key words : saturated fatty acid, non saturated fatty acid , L-carnitine, tuna, lemuru fish oils</em><em></em></p>

<p><em>The objective of this study were to find out and to compare the effect of supplementation of L-carnitine and tuna fish oil and lemuru fish oil on fatty acid saturated, fatty acid non saturated concentration and peroksida, TBA number.</em></p><p><em> The materials used were 20 male PE (Peranakan Etawah) goats of 2 until 3 month old and 15.5-23.5 kilogram body weight. The feedstuff used were wild grass, yellow corn, rice bran, soy bean meal, coconut meal, mineral premix. L-carnitine, tuna fish oil and lemuru fish oil. The method of the research was experimental in vivo using Completely Randomized Blok Design. There were 4 treatments in each treatment and 5 replication. Treatment consisted of P<sub>0</sub>= control ration, P<sub>1</sub>=P<sub>0</sub> +L-carnitine 50 ppm, P<sub>2</sub>=P<sub>1</sub> + tuna fish oil 5 ml or equal with 4% in the ration, and P<sub>3</sub>=P<sub>1</sub>+ lemuru fish oil 5 ml or equal with 4% in the ration. The variables measuared fatty acid saturated, fatty acid non saturated concentration and peroksida number..</em></p><p><em> The results of variance analysis showed that the effect of supplementation of tuna fish oil and lemuru fish oil (P<sub>2 </sub>and P<sub>3</sub>) in the rations contain 50 ppm L-carnitine were significantly (P<0.01) on faty acid saturated, faty acid non saturated concentration and peroksida number.</em></p><p><em>The conclusion of this research shows that the supplementation of 50 ppm L-carnitine, tuna and lemuru fish oil 5ml that was equal to 4% in goat rations can incrase non saturated fatty acid from 59,74 to 67,36%, than to decreased saturated fatty acid from 40,26 to 32,64 %. Proces burned of meat goat to increase peroxida from 0.1437 until 0.1615 miliequivalen/kg and malonaldehyde from 1,807 until 2,440 mg/kg then to decrease concentration omega-3 fatty acid from 5,957 until 5,945%.</em></p><p><em> </em></p><p><em>Key words : saturated fatty acid, non saturated fatty acid , L-carnitine, tuna, lemuru fish oils</em><em></em></p>

Lemuru fish (Sardinella longiceps) oil is a by-product containing polyunsaturated fatty acids (PUFA) that is important for the prevention of several diseases. For daily intake, saturated fatty acids should be removed from fish oil to obtain PUFA concentrates. Various methods have been observed in producing omega-3 concentrates. The objective of this study was to produce the omega-3 concentrate from lemuru fish oil using a two-step urea complexation method and to characterize the omega-3 product. Lemuru fish oil was saponified and purified to produce fatty acids and urea was added to the fatty acids to obtain crystal complexes. In this study, crystallization was carried out at -20 °C for 24 h with an oil-urea ratio of 1:0.625, 1:1.25, and 1:2.5. The results showed that the highest content of omega-3 (ALA, DHA, EPA) was reached at a ratio of fish oil-urea of 1:0.625. Based on the FTIR spectrum analysis, omega-3 concentrate contained the specific spectrum indicated by HC=CH trans, -C=O (ester), and -C-O (acid) groups. UV-Vis spectra analysis showed that light exposure affected the stability of omega-3 concentrate, as is evident by the hypsochromic shift of the resulting spectrum.

<p><em>The research was to know the influence of the supplementation lemuru fish oil and L-carnitine on the local male ducks’ performance. The cattle used were 96 local male ducks aged 10 days. Research materials were corn, bran, concentrate 144, lemuru fish oil, and L-carnitine. The research design used was Complete Random Design with 3 treatments and 8 repetitions; each repetition contained 4 local male ducks. The treatment consisted of ration control P0 = corn, barn, concentrate and mineral; P1 = corn, barn, mineral and 4% lemuru fish oil; P2 = corn, barn, concentrate, mineral, 4% lemuru fish oil and 30 ppm L-carnitine. The investigated modifiers were feed consumption, feed conversion, daily weight increase and Income Over Feed Cost value. Data feed consumption, daily weight increase, feed conversion was analyzed using variance analysis. If there were a concrete influence, further analysis should be conducted using orthogonal contrast. Results showed that the supplementation of lemuru fish oil and L-carnitine had a very significant influence (P<0.01) on feed consumption, daily weight increase and feed conversion. Further test results orthogonal contrast showed that the supplementation of lemuru fish oil and L-carnitine could decrease feed consumption, feed conversion and increase daily weight rate (P<0.01) if compared control. Result Income Over Feed Cost showed that treatment with add lemuru fish oil and L-carnitine produced Income Over Feed Cost value higher if compared control. Based on the results of the research, it could be concluded that the supplementation of 4% lemuru fish oil and 30 ppm L-carnitine in ration could decrease feed consumption and feed conversion, increase daily weight rate, and the IOFC of local male ducks.</em></p>

The aim of this study was to find out the effect of L-carnitine and fish oils on crude fat and fiber digestibilities, and feed conversion in native chicken. This study used 100 Kampung chickens aged 20 weeks. Feed treatments were divided into four groups consisted of basal diet, basal diet supplemented by L-carnitine, basal diet supplemented by L-carnitine and Tuna fish oil, and basal diet supplemented by L-carnitine and Lemuru fish oil. The data observed in this study were crude fat consumption and digestibility, crude fiber consumption and digestibility, feed consumption, and feed conversion ratio (FCR). Those data were analyzed using analysis of variance and an orthogonal contrast test was applied to distinguish among groups. Statistical analysis showed that highly significant effects of L-carnitine and fish oils supplementation on crude fat and fiber consumptions, and crude fat digestibility were found in this study (P<0.01). Moreover, L-carnitine and fish oils supplementation in the diet significantly affected crude fiber digestibility, feed consumption, and FCR (P<0.05). In conclusion, supplementation of Tuna and Lemuru fish oils until 4% containing 30 ppm L-carnitine improved crude fat consumption, digestibilities of crude fat and fiber, and decreased crude fiber digestibility, feed consumption, and FCR.

The content of EPA and DHA in lemuru fish oil can be used to prevent obesity risk factors. This study aims to determine the effect of purified lemuru fish oil on the weight of Wistar rats induced by trans fatty acids. This research is an experimental study using a randomized block design with a post test only control group. The research sample was 30 male Wistar rats aged two to three months, grouped into two control groups (K) which were given standard feed (K0) and those given standard feed after two weeks of trans fatty acid induction (K1) and three treatment groups (P). ) with lemuru fish oil intervention as much as 0.34 ml (P1), 0.67 ml (P2), and 1.34 ml (P3). The induction material was 2.3 g of liquid margarine, containing 3.8% trans fatty acids. The intervention material was lemuru fish oil from the purification process containing 18.05% EPA and 9.37% DHA. Induction of margarine for two weeks made the body weight of the rats increased by 27.90% (P2) compared to the control (K0) 16.06%. After the intervention period of lemuru fish oil for three weeks, the weight of the treatment group P1 (18.53%), P2 (14.74%), and P3 (13.19%) was lower than the control group K0 (22.79%) and K1 (25.31%).

nsaturated fatty acids (UFAs), particularly polyunsaturated fatty acids (PUFAs) such as oleic, linoleic, linolenic, arachidonic acids, EPA, and DHA, play a vital role in livestock productivity and in improving the nutritional quality of animal products. However, PUFAs are highly susceptible to biohydrogenation in the rumen, reducing their biological availability. The novelty of this study lies in evaluating lemuru fish oil protected with calcium hydroxide [Ca(OH)₂], which has not been specifically assessed in previous fat-protection research. This study aimed to determine the effect of unprotected and Ca(OH)₂-protected lemuru fish oil on the in vitro fatty acid profile. A nested randomized block design was applied under laboratory conditions, with treatments consisting of unprotected fish oil (P1) and Ca(OH)₂-protected fish oil (P2) at levels of L0 (0%), L5 (5%), L7.5 (7.5%), L10 (10%), and L12.5 (12.5%), each replicated three times. Variables observed included oleic, linoleic, linolenic, arachidonic acids, EPA, and DHA. Data were analyzed using analysis of variance (ANOVA) followed by Duncan’s multiple range test. Results indicated that both treatment type and supplementation level had highly significant effects (P<0.01) on fatty acid profiles. The highest lipid concentrations were achieved with 10% supplementation of protected fish oil, while the best outcome for unprotected oil occurred at the 5% level. These findings provide practical implications for livestock feeding, showing that unprotected lemuru fish oil can be effectively utilized up to 5% inclusion, whereas Ca(OH)₂-protected lemuru fish oil can be safely applied up to 10%, offering a sustainable strategy to improve ruminant productivity and product quality.

<p><span><em>The aim of the research was to evaluate the effect of feeding fermented yellow corn with </em><span><em>polyunsaturated fatty acid and L-Karnitin supplementation on the hatchability of quail (Coturnix-coturnix </em><span><em>japonica) egg. A number of 160 female and 40 male quails of 70 days old were used as experimental </em><span><em>animal. The present research was designed in oneway Completely Randomized Design (CRD) with five </em><span><em>treatments and four replication, each replication contains of eight female and two males quails. The </em><span><em>dietary treatments were P0= basal diet, P1= P0 with 100% substitution at yellow corn by fermented yellow </em><span><em>corn, P2= P1 + 10 ppm L-karnitin, P3= P2 + 4% tuna fish oil, P4= P2 + 4% lemuru fish oil. Suplementation </em><span><em>of fish oil as polyunsaturated fatty acid source and L-karnitin in fermented yellow corn diet didn’t affect </em><span><em>eggs fertility, hatchability, and hatching quality, however, it significantly (P<0.01) affected hatching weight. </em><span><em>The best result achieved in treatment P1 because it content yellow corn fermentation which increased the </em><span><em>weight of hatching. It can be concluded that feeding fermented yellow corn with supplementation of </em><span><em>polyunsaturated fatty acid source and L-karnitin quail eggs, increase DOQ hatching weight of quail eggs.</em><br /></span></span></span></span></span></span></span></span></span></span></span></span></p><p><span><span><span><span><span><span><span><span><span><span><span><span><span><em>(Key words: Fermented yellow corn, Hatchability, L-karnitin, Polyunsaturated fatty acid, Quail)</em></span></span></span></span></span></span></span></span></span></span></span></span><br class="Apple-interchange-newline" /></span></p>

This study was conducted to determine the effect of ipil ipil and bean leaves supplementation on egg production, egg quality and growth performance of Japanese quail. A total of 40 “Japanese Quail” at the age of 42 days (06 weeks) old were divided into four groups T0, T1, T2 and T3 respectively. Group T0 was considered as control which fed only with commercial layer ration. Group T1 was supplemented with formulation of 2 gm grinded ipil ipil leaves, Group T2 with 2 gm grinded Bean leaves and Group T3 with 2 gm grinded ipil ipil leaves plus 2 gm grinded Bean leaves per kg feed respectively. Observations were recorded for growth performance, egg production and egg quality of quail. Increased egg production rate was observed in ipil ipil supplemented groups (group T1). Body weights were increased significantly (p<0.05) in all treated groups in respect to the control and highest was recorded in combined ipil ipil and Bean leaves supplemented groups (Group T3). In treatment groups, there were significant increase in egg production, but in case of control group, the egg production was not increased. There was no significant pathological change in any internal organs of the layer of treated groups. Best result was found in the group T3. The present study reveals that combined supplementation of ipil ipil and bean leaves gives better result over the other groups in respect to growth performance, egg production and egg quality.Asian J. Med. Biol. Res. June 2018, 4(2): 201-206

The present study was conducted to evaluate the effect of Sauropus androgynus (katuk) leavesextract (SAE) and lemuru fish oil (LO) on fat deposition and fatty acid composition of meat in broilerchickens. One hundred and fifty six broiler chickens were distributed to 13 treatment groups with 3cages in each treatment group as replicate. Completely randomized design was used in this study. Thethirteen groups were subsequent of broiler chickens that were fed diet containing commercial feedsupplement as a control (P1), 10 g/kg SAE and 1% LO (P2); 10 g/kg SAE and 1% LO plus 60 mgvitamin E (P3), 10 g/kg SAE and 2% LO (P4), 10 g/kg SAE and 2% LO plus 60 mg vitamin E (P5), 10g/kg SAE and 3% LO (P6), 10 g/kg SAE and 3% LO plus 60 mg vitamin E (P7), 18 g/kg SAE and 1%LO (P8), 18 g/kg SAE and 1% LO plus 60 mg vitamin E (P9), 18 g/kg SAE and 2% LO (P10), were18 g/kg SAE and 2% LO plus 60 mg vitamin E (P11), 18 g/kg SAE and 3% LO (P12), and 18 g/kg SAEand 3% LO plus 60 mg vitamin E (P13). The data were analyzed by analysis of variance and if it weresignificant, it were then determined by Duncan's Multiple Range test. The present results showed thatsupplementation of SAE and LO significantly affected (P<0.05) fat deposition in abdomen and leg, butit had no effect on neck fat deposition and Fatty Liver Score. Supplementation of SAE and LO hadsignificantly reduced (P<0.05) cholesterol content and thiobarbituric acid (TBA) in leg meat, but itsignificantly increased (P<0.05) vitamin A and vitamin E and it had no effect on fat in leg meat.Supplementation of SAE and LO proved to change fatty acid composition in leg meat. The treatmenthighly significant increased ecosapentaenoic acid (EPA) and docosahexaenoic acid DHA (P<0.01),arachidonic acid and stearic acid (P<0.05), but it significantly reduced linolenic acid (P<0.05). Inconclusion, the supplementation of SAE and LO reduced fat deposition in abdomen and leg, the contentof cholesterol in meat, and it enriched EPA and DHA of meat.

The research aimed to investigate the effect of cellulase, carnitine and Lemuru fish oil supplementation on lipid and fatty acid contents of Muscovy duck eggs. A total of 120 Muscovy duck was allocated randomly to 4 dietary treatments. The basal diet consisted of corn, rice bran and layer concentrate, defined as P1. The remaining treatments were basal diet + 0.1% cellulase enzyme (P2), basal diet + 0.1% cellulase enzyme + 40 ppm L carnitine (P3), basal diet + 0.1% cellulase enzyme + 40ppm L-carnitine + 4% Lemuru fish oil (P4). Result shows supplementation of cellulase enzyme did not influence lipid, cholesterol, and fatty acid contents in eggs resulted, while supplementation of carnitine decreased cholesterol content (P<0.01) without affecting lipid and fatty acid contents. Furthermore, supplementation of Lemuru fish oil in diet containing cellulase enzyme and carnitine enhanced lipid, high-density lipoprotein, and unsaturated fatty acid contents in the eggs (P<0.01). Accordingly, supplementation of Lemuru fish oil decreased low-density lipoprotein, cholesterol, and saturated fatty acid contents (P<0.01). It is concluded that supplementation of carnitine and fish oil in the diet produced Muscovy duck eggs with considerably high unsaturated fatty acids and low cholesterol contents.

The research was conducted to investigate the effect of supplementation of cellulase, carnitine and tuna fish oil on lipid and fatty acid contents of Indonesian native chicken meats. A total of 120 male native chickens was allocated randomly to 4 dietary treatments with 6 replicates of 5 birds. The basal diet, which was consisted of corn, rice bran and layer concentrate, was defined as T1. The remaining 3 dietary treatments were the basal diet + 0.1% cellulase enzyme (T2), basal diet + 0.1% cellulase enzyme + 30 ppm L-carnitine (T3), basal diet + 0.1% cellulase enzyme + 30 ppm L-carnitine + 4% lemuru fish oil (T4). The dietary treatments were fed for 90 days. Supplementation of cellulase enzyme did not influence the lipid, cholesterol and fatty acid contents in the meats. Supplementation of carnitine in the diet containing cellulase enzyme decreased cholesterol content (P<0.05) without affecting lipid and fatty acid contents in the meats. Furthermore, supplementation of lemuru fish oil in the diet containing cellulase enzyme and carnitine enhanced the lipid, high-density lipoprotein and unsaturated fatty acid contents, including omega-3 and omega-6 fatty acids in native chicken meats (P<0.05). Accordingly, supplementation of lemuru fish oil decreased the low-density lipoprotein, cholesterol and saturated fatty acid contents in the meats (P<0.05). It is concluded that the supplementation of carnitine and fish oil in the diet produced native chicken meats with considerably low cholesterol and high unsaturated fatty acids contents.

Moina macrocopa is a natural food that has the potential for freshwater fish seeds, because of its high nutritional value, easy to digest and has a fast, fast breeding, and has a size that is in accordance with the fish's mouth opening. Problems in the use of M. macrocopa as natural food are constrained by the availability of M. macrocopa both from nature and from cultivation. Population density in M. macrocopa cultivation varies due to different quality of food used. The needs of M. macrocopa as natural food can be met if the quality and quantity of feed used optimally support population growth. This study aims to determine the effect of lemuru fish oil concentration in bran suspension fermentation on fecundity and offspring production of M. macrocopa. This study used a completely randomized design (CRD) consisting of five treatments three replications, namely: control treatment (0 mg/L lemuru fish oil, 100 gram bran), P1 (4 mg/L lemuru fish oil, 100 gram bran), P2 (lemuru fish oil 5 mg/L, 100 gram bran ), P3 (lemuru fish oil 6 mg/L, 100 gram bran), P4 (7 mg/L lemuru fish oil, 100 grams), and P5 (8 mg/L lemuru fish oil, 100 gram bran ).The parameters observed were fecundity and child production per parent and water quality. Data analysis used the Analysis of Variance test followed by Duncan's multiple test. The results showed that the cultivation of M. macrocopa using different bran suspension feed with supplemented lemuru fish oil affected the fecundity and production of children per M. macrocopa parent. The concentration of lemuru fish oil of 6 mg/L in the feed resulted in a fecundity of 24,00±2,00 eggs/parent and child production is 20,33±1,52 ind/parent.

The aim of this research was to evaluate the use of lemuru fish oil as palm oil substitution in ration on broiler performance. This research used 200 Ross broilers day old chick (DOC) is divided into 4 treatments and 5 replications. This research used a completely randomized design (CRD), where the treatments level of palm oil and lemuru fish oil were respectively P0 = rations containing 3% palm oil and 0% lemuru fish oil, P1 = rations containing 2% palm oil and 1% lemuru fish oil, P2 = rations containing 1% palm oil and 2% lemuru fish oil, P3 = rations containing 0% palm oil and 3% lemuru fish oil. The variables observed were feed intake, final body weight, body weight gain, feed conversion ratio (FCR), mortality, and income over feed and chick cost (IOFCC). The data were analyzed using analysis of variance (ANOVA). The significant result (P&lt;0,05) were followed by Duncan test. The results showed a significant effect (P&lt;0.05) in decreasing FCR in the starter period with a higher level of use of lemuru fish oil, increasing body weight gain and decreasing FCR in the finisher period with a lower level of use of lemuru fish oil, but the treatments did not significantly effect on performance broiler during the age of 35 days. Weight and percentage of internal organs and digestive tract under normal conditions, except liver weight.&#x0D; The conclusion of this study was the use of 3% lemuru fish oil can improve FCR in the starter phase, but the different level of palm oil and lemuru fish oil until 3 % in broiler rations does not affect on broiler performance during the age of 35 days.&#x0D;

The purpose of this study was to evaluate the effect of giving levels of secang wood extract and lemuru fish oil on the appearance of laying quails which included feed consumption, Hen Day Production (HDP), feed conversion of quail egg mortality. This study used Autumn strain quail with a population of 168 individuals with basal feed supplemented with several levels of secang wood extract. This research method is an experiment using a completely randomized design (CRD) factorial with 4 treatments with 6 replications with each repetition consisting of 7 quails aged 10-15 weeks. The treatment was as follows: P0 = basal feed without the addition of secang wood extract P1 = basal feed + 0.2% EKS + 4% MIL, P2 = basal feed + 0.4% EKS + 4% MIL, P3 = basal feed + 0 , 6% EX + 4% MIL. The addition of a combination of secang wood extract and fish oil was carried out according to the level of quail feeding. The variable being measured; feed consumption, Hen Day Production (HDP), Feed conversion, mortality. Addition of secang wood extract with a level of 0%; 0.2%; 0.4%; 0.6%, and 4% lemuru fish oil had no significant effect on feed consumption, hen day production, feed conversion, mortality, this was due to flavonoids, polyphenols and active compounds in oil. lemuru fish does not affect the appearance of quail egg production. Flavonoid compounds in low concentrations have no effect on chicken feed consumption, because the content of flavonoid compounds can still be tolerated by the chicken body. The use of lemuru fish oil with a concentration of up to 6% in quails does not affect the consumption of quail feed, because the addition of lemuru fish oil does not affect the palatability of quail feed. The conclusion of this study was that the addition of secang wood extract. The addition of secang wood extract and lemuru fish oil did not affect hen day production, feed conversion and mortality of laying quails