Indomethacin-induced gastrointestinal ulcers in rats: Effects of dietary fatty acids and endotoxin

Abstract

The purpose of this study was to determine how dietary n-3 and n-6 polyunsaturated fatty acids (PUFA) affected the gastrointestinal response to lipopolysaccharide (LPS) and indomethacin (INDO) in the rat. Rats were fed diets containing 12.5% linseed oil (LO — enriched in short-chain n-3 PUFA) or corn oil (CO — enriched in n-6 PUFA). After 30 days on the diets, rats were given one of three treatments 1: 10 mg/kg O55:B5 Escherichia coli LPS intraperitoneally (i.p.), 2: 25 mg/kg INDO subcutaneously (s.c.), 3: a combination of 10 mg/kg i.p. LPS and 25 mg/kg (s.c.) INDO given 30 min before LPS. 20 h after challenge, rats were given an intravenous injection of Monastral Blue B to stain ulcer areas in the gastrointestinal tract. Lipopolysaccharide did not result in any Monastral Blue B vascular leakage in the gastrointestinal tract. Rats on the LO diet had significantly increased stomach and intestinal ulcers compared to CO fed rats. When rats were challenged with LPS and INDO, the LPS almost completely eliminated small intestinal ulcers, but enhanced ulcer development in the stomach.