Indole-3-butyric acid and myo-inositol impacts on in vitro rooting of the cherry rootstocks CAB-6P and Gisela 6

Abstract

The present study investigates the effects of indole-3-butyric acid (IBA) alone and in combination with myo-inositol on in vitro rooting and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens). For the CAB-6P rootstock, the best results for root number (6.31), fresh mass (FM), dry mass (DM), and rooting percentage (100 %) were obtained on Murashige and Skoog (MS) medium with 2 mg dm-3 IBA and maximum root length (30.57 mm) was obtained at 1 mg dm-3 IBA. Myo-inositol suppressed the positive effects of IBA on root length. In the Gisela 6 explants, the inclusion of 2 mg dm-3 IBA together with 0.5 mg dm-3 of myo-inositol in the culture medium significantly enhanced root number (9.91) and root FM and DM. The root length was maximum in the combination of the lowest IBA and myo-inositol concentrations (0.5 mg dm-3). The rooting percentage was the greatest (100 %) with the application of 1 mg dm-3 IBA alone. In both explants, the application of IBA alone or in combination with myo-inositol resulted in a lower leaf proline content in comparison with the control (without growth regulators). The maximum leaf chlorophyll content was at 1 mg dm-3 IBA in the CAB-6P whereas at 2 mg dm-3 IBA and 1 mg dm-3 myo-inositol in Gisela 6. Addition of myo-inositol mostly increased sugar content in comparison with control or IBA alone in both rootstocks.