Indole acetic acid (IAA) hormone production by endophytic bacteria isolate from Cinchona plant (Cinchona ledgerina Moens.) root

Abstract

Current agricultural production depends on the large-scale use of chemical fertilizers. However, excessive use of fertilizer can cause harmful effects on the environment. Against this issue, current agricultural practice is moving towards a more sustainable and environmentally friendly approach. Modern strategies increasing agricultural productivity emphasize the importance of biofertilizers in realizing their objective. Endophytic bacteria is known as the most promising and effective biofertilizers. Various types of endophytes and their potential can be obtained from various plants and studied to determine the potential in promoting plant growth. This study aims to determine the Cinchona plant (Cinchona ledgeriana Moens.) root's endophytic bacteria in producing Indole Acetic Acid (IAA) hormone and analyze its concentration IAA hormone production. IAA-producing bacteria activity test is done qualitatively and quantitatively. IAA assay was measured by using spectrophotometry technique with a wavelength of 530 nm. The results showed that all 23 isolates of the Cinchona plant endophytic bacterial roots could produce IAA hormone. The hormone production from 23 bacterial isolates showed that the average IAA production amount 10.60 ppm. The results showed that isolates a15 able to produce the highest IAA amount of 68.35 ppm.